Physiological responses of C4 grasses to prolonged heat stress

C4 grasses are best adapted to the transition, warm-arid, and warm-humid climatic zones and have the ability to acquire thermotolerance by exposure to acute heat stress. Exposure to sub-lethal temperatures results in changes in physiological, biochemical, metabolic, and molecular processes. The response of two warm-season grasses to prolonged heat stress was investigated. Plants of hybrid bermudagrass (Cynodon dactylon × C. transvaalensis ‘Tifway’) and Japanese lawn grass (Zoysia japonica Steud. ‘Meyer’) were exposed for 168 h to supraoptimal temperature conditions (47°C) in controlled-environment chamber. Compared with zoysiagrass, bermudagrass showed greater damage. Metabolite profi les were affected by prolonged heat exposure, with signifi cant differences between these species. Consistent differences were found in total soluble sugars accumulation over the study period and severity of plant organ senescence. Bermudagrass roots were more affected, as compared to leaves. Leaf proteins expression determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis showed an early degradation in zoysiagrass, as thermal exposure proceeded. A signifi - cant net decline in protein content was observed after 48 h of exposure, while in bermudagrass an analogous decline was not detected until 96 h of treatment. Although heat stress is not considered a detrimental factor to C4 grass species, the two species showed signifi cant differences in their physiological response to continuous high temperatures

Winter Colour Retention and Spring Green-Up of Zoysiagrass Genotypes in Southern Europe

Zoysiagrass (Zoysia spp. Wild.) is a sustainable choice for the Mediterranean area, being identified as grass species adapted to low-input environments (limited water requirements, no fertilizer or pesticides after establishment). A distinct drawback in the transition zone is winter dormancy, a temporary suspension of visible growth, followed by discoloration and loss of functionality. In order to investigate the genotype/species effect in relation with agro-meteorological conditions, 10 cultivars/experimental lines were screened for their ability to extend the winter colour retention. A two years field study was conducted on mature swards and evaluated during the onset of dormancy and spring green-up using digital image analysis. For both parameters, in both years, the Authors observed a wide range of cultivar responses. Zoysia japonica genotypes in our study provided significantly shorter dormancy period than fine-leaved genotypes. In some instances, there were as much as 35 and 20 d difference between entries in respect to the winter colour retention and spring onset, respectively. The identification of genotypes with shorter dormancy than 'Meyer' (industry standard) may reduce use of cultural practices, as late-season nitrogen or iron application, normally used to extend the winter colour retention of zoysiagrass in late fall or early spring, showing different levels of success

Carbohydrate content, characterization and localization in bermudagrass stolons during establishment

Abstract Turfgrasses are continuously exposed to a wide range of detrimental stresses, such as mowing, traffic, low or high temperatures, salinity, drought, UV, hypoxia etc. Plant responses to stimuli involve nearly every aspect of plant physiology and metabolism. Carbohydrates, primary sucrose and starch, as principal metabolic products of photosynthesis, are stored in bermudagrass (Cynodon spp.) in stolons and rhizomes. Total nonstructural carbohydrates (TNC) levels have been used as indicators of health and physiological status in bermudagrass. TNC levels vary during the year and are weakly affected by N source. Establishment is a critical phase of bermudagrass management. The objectives of this research were to assess and characterize stolon carbohydrate content in nine bermudagrass cultivars during the full establishment phase in relation to establishment rates. Morphological and growth analyses showed distinct properties among the cultivars selected for this study. Significant differences in turf co..

Carbohydrate Metabolism During Wintering Period in Four Zoysiagrass Genotypes

A key barrier to widespread use of warm-season grasses in the transition zone between the temperate and subtropical climates is represented by the winter dormancy, a temporary suspension of visible growth of any plant structure including the meristem. In this environment, species with different photosynthetic carbon cycle coexist, leading to asynchrony in growth among warm- and cool-season grasses. The objectives of the present study were (i) to assess the carbohydrate content in four representative zoysiagrasses Zoysia japonica 'El Toro', 'Meyer', Z. pacifica, and Z. matrella 'Zeon' during three successive phases of vegetation phenology, senescence, dormancy, and green-up, and (ii) to characterize the metabolic responses to phenological variations under natural acclimation. Japanese lawn grass genotypes 'El Toro' and 'Meyer' showed longer dormancy periods than fine-leaved Z. pacifica and 'Zeon'. In Japanese lawn grass genotypes, the progressive decline of photosynthetic pigments was similar to that observed in the color retention. Over the experimental period, a significantly greater amount of starch reserves was observed in sprigs (horizontal stems) in the Japanese lawn grasses than in the fine-leaved zoysiagrasses. In general, total soluble sugars (TSS) in leaves and sprigs did not show evidence of sugar starvation during the senescence phase. TSS increased significantly during cold acclimation, resulting in a higher metabolic activity at the onset of green-up to support spring regreening in all zoysiagrasses except 'Meyer' leaves

Warm-season turfgrass species generate sports surfaces with different playability

Synthetic sports surfaces are increasingly subject to standardization of athlete-surface and ball-surface interactions (playability parameters). Such standardizations have led to an increase in the level of the engineering and predictability of these surfaces, and as such may be beneficial also for natural turf. In warm and temperate climates, many natural turf sports surfaces are established with warm-season (C4) turfgrass species due to their suitability to the environment in such areas. This study was aimed at evaluating the Féderation Internationale de Football Association (FIFA)-standard playing characteristics of different sports turf surfaces obtained from three commonly used C4 turfgrass species: 1) ‘Tifway 419’ hybrid bermudagrass (Cynodon dactylon var. dactylon × C. transvaalensis), 2) ‘Zeon’ manilagrass (Zoysia matrella), and 3) ‘Salam’ seashore paspalum (Paspalum vaginatum) for factors concerning leaf tissue (silica, lignin, water content) and canopy structure (shoot density, leaf architecture, stolon density, etc.). Results showed that surfaces of different C4 turfgrass species generate different playability parameters, with seashore paspalum being a harder faster surface, manilagrass being a softer slower surface, and hybrid bermudagrass showing intermediate characteristics. These playing quality results were associated with certain specific canopy biometrical/morphological parameters such as shoot density, horizontal stem density (HSD), leaf section, and, to a lesser extent, to certain plant tissue compounds (lignin, silica)

Seedling Establishment of Tall Fescue Exposed to Long-Term Starvation Stress

In germinating seeds under unfavorable environmental conditions, the mobilization of stores in the cotyledons is delayed, which may result in a different modulation of carbohydrates balance and a decrease in seedling vigor. Tall fescue (Festuca arundinacea Schreb.) caryopses grown at 4˚C in the dark for an extended period in complete absence of nutrients, showed an unexpected ability to survive. Seedlings grown at 4˚C for 210 days were morphologically identical to seedlings grown at 23˚C for 21 days. After 400 days, seedlings grown at 4˚C were able to differentiate plastids to chloroplast in just few days once transferred to the light and 23˚C. Tall fescue exposed to prolonged period at 4˚C showed marked anatomical changes: cell wall thickening, undifferentiated plastids, more root hairs and less xylem lignification. Physiological modifications were also observed, in particular related to sugar content, GA and ABA levels and amylolytic enzymes pattern. The phytohormones profiles exhibited at 4 and 23˚C were comparable when normalized to the respective physiological states. Both the onset and the completion of germination were linked to GA and ABA levels, as well as to the ratio between these two hormones. All plants showed a sharp decline in carbohydrate content, with a consequent onset of gradual sugar starvation. This explained the slowed then full arrest in growth under both treatment regimes. The analysis of amylolytic activity showed that Ca2+ played a central role in the stabilization of several isoforms. Overall, convergence of starvation and hormone signals meet in crosstalk to regulate germination, growth and development in tall fescue

Autonomous rotary mower versus ordinary reel Mower-effects of cutting height and nitrogen rate on manila grass turf quality

High-quality sports turfs often require low mowing and frequent maintenance. Sports turfs often consist of hard-to-mow warm season turfgrasses, such as zoysiagrass (Zoysia sp.) or bermudagrass (Cynodon sp.). Although autonomous mowers have several advantages over manually operated mowers, they are not designed to mow lower than 2.0 cm and are consequently not used on high-quality sports turfs. All autonomous mowers are only equipped with rotary mowing devices and do not perform clipping removal. An ordinary autonomous mower was modified to obtain a prototype autonomous mower cutting at a low height. The prototype autonomous mower was tested on a manila grass (Zoysia matrella) turf and compared its performance in terms of turf quality and energy consumption with an ordinary autonomous mower and with a gasoline reel mower. A three-way factor experimental design with three replications was adopted. Factor A consisted of four nitrogen rates (0, 50, 100, and 150 kghaL1), factor B consisted of two mowing systems (autonomous mower vs. walk-behind gasoline reel mower with no clipping removal), and factor C consisted of two mowing heights (1.2 and 3.6 cm). Prototype autonomous mower performed mowing at 1.2-cm mowing height whereas ordinary autonomous mower mowed at 3.6-cm mowing height. The interaction between the mowing system and mowing height showed that the turf quality was higher when the turf was mowed by the autonomous mower and at 1.2 cm than at 3.6 cm. Autonomous mowing not only reduced the mowing quality, but also reduced the leaf width. Lower mowing height induced thinner leaves. Nitrogen fertilization not only increased the overall turf quality, reduced weed cover percentage, but also reduced mowing quality. Autonomous mowers also had a lower energy consumption if compared with the reel mower (1.86 vs. 5.37 kWh/ week at 1.2-cm mowing height and 1.79 vs. 2.32 kWh/week at 3.6-cm mowing height, respectively). These results show that autonomous mowers can perform low mowing even on tough-to-mow turfgrass species. They could also be used on highquality sports turfs, thus saving time as well as reducing noise and pollution

Use of Flaming to Control Weeds in ‘Patriot’ Hybrid Bermudagrass

Flaming could be an alternative to the use of chemical herbicides for controlling weeds in turfgrass. In fact, the European Union has stipulated that chemical herbicides should be minimized or prohibited in public parks and gardens, sports and recreational areas, school gardens, and children’s playgrounds. The aim of this research was to test different doses of liquefied petroleum gas (LPG) to find the optimal flaming dose that keeps a ‘Patriot’ hybrid bermudagrass (Cynodon dactylon · Cynodon transvaalensis) turf free of weeds during spring green-up, but also avoids damaging the grass. Five LPG doses (0, 29, 48, 71, and 100 kgha–1) were applied in a broadcast manner over the turf experimental units using a selfpropelled flaming machine. This equipment is commercially available and usable by turfgrass managers. Treatments were applied three times during the spring to allow the maximum removal of weeds from the turfgrass. Data on weed coverage, density, biomass, and turfgrass green-up were collected and analyzed. Results showed that 3 weeks after the last flaming, the greatest LPG doses used (i.e., 71 and 100 kgha–1) ensured the least amount of weeds (range, 5–16 weeds/m2) of low weight (range, 7– 60 gm–2) and a low weed cover percentage (range, 1% to 5%), whereas the green turfgrass coverage was high (range, 82% to 94%). At the end of the experiment, the main weed species were horseweed (Conyza canadensis), field bindweed (Convolvulus arvensis), narrow-leaved aster (Aster squamatus), and black medic (Medicago lupulina). Flame weed control is a promising technique to conduct weed control in turfgrass. Further studies could be conducted to investigate the use of flaming in other species of warm-season turfgrasses

Spectral Reflectance of Tall Fescue (Festuca Arundinacea Schreb.) Under Different Irrigation and Nitrogen Conditions

The issue of water and climate change is present in many countries. Drought stress is one of the main abiotic stresses influencing turfgrass growth and quality. Tall fescue is the most suitable cool-season turfgrass for the Mediterranean region. This species has a better heat tolerance than perennial ryegrass and Kentucky bluegrass. The analysis of radiation reflected by turfgrass can supply precious information on drought stress and nutritional status. In this study a Linear Gradient Irrigation System (LGIS) was adopted on a Festuca arundinacea turf with 9 water replenishment levels and 2 nitrogen conditions, to evaluate the proximity sensed spectral reflectance. ET0 was estimated using the Hargreaves and Samani method. The following parameters were determined: turf quality, drought tolerance, pest problems, temperature of the surface, clippings weight and relative nitrogen content, turf growth and soil moisture. Spectral reflectance data were acquired using a LICOR 1800 spectroradiometer. Pearson correlation coefficients were studied among all parameters and vegetation indices. Nitrogen fertilization influenced significantly turf quality, clippings weight, nitrogen content and turf growth. Water replenishment influenced significantly all parameters except nitrogen content. Among all parameters the highest correlation coefficient was registered relating drought tolerance with turf quality (r = 0.88) and with surface temperature (r = - 0.88). Among vegetation indices results showed that Water Index (WI) and Normalized Difference Water Index (NDWI), are the most suitable to discriminate between different levels of water replenishment. Comparing WI with NDWI, the correlation coefficients were higher for Water Index in all the parameters, in particular the highest WI value was registered for drought tolerance (r = 0.91). This preliminary research demonstrates that spectral remote sensing can be a useful diagnostic tool to detect water stress in turfgrasses

Steaming and flaming for converting cool-season turfgrasses to hybrid bermudagrass in untilled soil

Turfgrass species can be classified into two main groups: cool-season and warm-season species. Warm-season species are more suited to a Mediterranean climate. Transplanting is a possible method to convert a cool-season to a warmseason turfgrass in untilled soil. It generally requires the chemical desiccation of the cool-season turfgrass. However, alternative physical methods, like flaming and steaming, are also available. This paper compares flaming, steaming, and herbicide application to desiccate cool-season turfgrass, for conversion to hybrid bermudagrass (Cynodon dactylon x C. transvaalensis) in untilled soil, using transplanting. Two prototype machines were used, a self-propelled steaming machine and a tractor-mounted liquefied petroleum gas flaming machine. Treatments compared in this work were two flaming treatments and two steaming treatments performed at four different doses together with two chemical treatments with glufosinateammonium herbicide applications. The cool-season turfgrass species were tall fescue (Festuca arundinacea) and perennial ryegrass (Lolium perenne). The desiccation effect of the various treatments on cool-season turf was assessed by photographic survey 15 days after treatment. The percentage cover of hybrid bermudagrass was visually assessed at 43 weeks after planting. Steaming and flaming effects on both parameters were described by logistic curves. The highest doses of steaming and flaming almost completely desiccated cool-season turf, and similar hybrid bermudagrass cover was established by both the methods as the chemical application (50% to 60%). Thus both flaming and steaming may be considered as valid alternatives to herbicides aimed at turf conversion